<p>DNA ligase (polydeoxyribonucleotide synthase) is the enzyme that joins two DNA fragments by catalyzing the formation of an internucleotide ester bond between phosphate and deoxyribose. It is active during DNA replication, DNA repair and DNA recombination. There are two forms of DNA ligase: one requires ATP (<db_xref db="EC" dbkey="6.5.1.1"/>), the other NAD (<db_xref db="EC" dbkey="6.5.1.2"/>).</p><p>This family is predominantly composed of NAD-dependent bacterial DNA ligases. They are proteins of about 75 to 85 Kd whose sequence is well conserved [<cite idref="PUB00001728"/>, <cite idref="PUB00002786"/>]. They also show similarity to yicF, an <taxon tax_id="562">Escherichia coli</taxon> hypothetical protein of 63 Kd.</p> NAD-dependent DNA ligase